sk mel 28  (ATCC)

Journal: bioRxiv

Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer

doi: 10.64898/2026.01.16.699897

Figure Lengend Snippet: (A) Hybridomas generated from the top five anti-sera were evaluated for specificity using western blot analysis of full-length recombinant GST-DRP1 ± ERK1 treatment. 100 ng of recombinant GST-DRP1 was loaded per lane. Total DRP1 was evaluated for equal protein loading. (B–C) The hybridomas were screened using western blot analysis of whole cell lysates (100 µg/lane; B ) or heavy membrane fractions (25 µg/lane; C ) isolated from SKMEL28 and A375 cells treated with GSK1120212 (50 nM; GSK), PLX4032 (1 µM; PLX), or DMSO for 6 hours. Total DRP1 was evaluated for equal protein loading. (D) Primary melanoma lines were treated with GSK1120212 (50 nM) or PLX4032 (1 µM) for 6 hours. Whole cell lysates were western blotted for indicated proteins. Recombinant 3G11 was evaluated for DRP1-S616Ⓟ detection. Actin was probed for equal protein loading. (E) A375 and SKMEL28 were treated with GSK1120212 (50 nM) or PLX4032 (1 µM) for 6 hours and evaluated as in D . Recombinant 3G11 was evaluated for DRP1-S616Ⓟ detection. Actin was probed for equal protein loading. (F) Heavy membrane fractions from the same treatments in D were western blotted for the indicated proteins. Recombinant 3G11 was evaluated for DRP1-S616Ⓟ. HSP60 was probed for equal protein loading. Molecular weight standards for all western blots are indicated in kilodaltons (kDa). Data are representative of 3 independent experiments.

Article Snippet: A375 and SKMEL28 human-derived non-primary ( i.e., lymph node and secondary metastasis derived, respectively) melanoma lines were purchased from ATCC and cultured in DMEM media.

Techniques: Generated, Western Blot, Recombinant, Membrane, Isolation, Molecular Weight

Journal: bioRxiv

Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer

doi: 10.64898/2026.01.16.699897

Figure Lengend Snippet: (A) SKMEL28 cells were treated with GSK1120212 (50 nM) for 16 hours, fixed, and stained for TOMM20 (green; mitochondria) and Hoechst 33342 (blue; nuclei). Scale bars = 10 microns. (B) SKMEL28 cells were studied as in A . Mitochondrial networks are shown as maximum intensity projections of super-resolution Leica LIGHTNING deconvolved confocal z-stacks. Imaris surface features were used to generate 3D projections to visualize changes in mitochondrial network architecture. Scales bars = 5 microns. (C) Mitochondrial elongation was quantified using the Perimeter:Area Ratio. (D–E) SKMEL28 cells were treated with GSK1120212 (50 nM) for 16 hours, fixed, and stained for TOMM20 (green), DRP1-S616Ⓟ (red), and Hoechst 33342 (blue). Red channel intensity was quantified to assess DRP1-S616Ⓟ signal intensity using Mander’s coefficient. Scale bars = 10 microns. Zoomed network images = 50 micron scale bars. (F–G) A375 cells were analyzed using the same treatment, staining, imaging, and quantification approach as panels D–E . All experiments were performed at least twice, with statistical significance determined from a minimum of two independent experiments using a one-way ANOVA test.

Article Snippet: A375 and SKMEL28 human-derived non-primary ( i.e., lymph node and secondary metastasis derived, respectively) melanoma lines were purchased from ATCC and cultured in DMEM media.

Techniques: Staining, Imaging

Journal: Frontiers in Cell and Developmental Biology

Article Title: PTEN enhances the radiosensitivity of melanoma by inhibiting DNA-PKcs

doi: 10.3389/fcell.2025.1712429

Figure Lengend Snippet: Expression of PTEN in different tissues and cells. (A) Expression of PTEN in melanoma tissue and normal tissue analyzed using TCGA database. (B,C) PTEN expression in melanoma tissues was markedly decreased compared to that in adjacent non-cancerous tissues ( **** p < 0.0001). (D) TLR4 expression in melanoma cells (SK-MEL-28, SK-MEL-5, and A375) was relatively lower than that in normal skin cell lines (NHEK and HaCaT). (E) Quantitative analysis of . (F) Expression of PTEN in A375 and SK-MEL-28 cells at different time points after IR.

Article Snippet: Human melanoma cell lines SK-MEL-28, SK-MEL-5, and A375 and human keratinocyte cell lines HaCaT and NHEK cells were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing